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KMID : 0903519740170020125
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1974 Volume.17 No. 2 p.125 ~ p.137
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Studies on Glycolipids in Bacteria
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Abstract
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The chemical structure of glycolipid of Selenomonas ruminantium cell wall was to be elucidated. The bacterial cells were treated in hot TCA and the glycolipid fractions were extracted by the solvent CHCl₃: CH₃OH (1 : 3). The extracted glycolipids fraction was further separated by acetone extraction. The acetone soluble fraction was named as the spot A-compound. The acetone insoluble but ether soluble fraction was named as the spot B-compound. These two compounds were examined for elucidation of their chemical structure. The results were as follows:
1. The IR spectral analysis showed that O-acyl and N-acyl fatty acids were linked to glucosamine moiety in the spot A-compound. However in the spot B-compound in addition to O and N-acyl acids phosphorus was shown to be attached to glucosamine.
2. It was recognized by gas liquid chromatography that spot A compound contained beta-OH C_(13:0) fatty acid in predominance in addition to the fatty acid with beta-OH C_(9:0), whereas the spot B compound was composed of the predominant fatty acid of beta-OH C_(13:0) with small amount of beta-OH C_(9:0).
3. According to the paper chromatographic analysis of hydrazinolysis products of the spot A compound, a compound of a similar Rf value as the chitobiose was recognized, which indicated a structure of two molecules glucosamine condensed. The low Rf value of the hydrazinolysis product of the spot B-compound confirmed the presence of phosphorus attached to glucosamine.
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